FuGENE® 6 Transfection Reagent Reference: F6-1000 FuGENE® 6 is a multi-component lipid-based transfection reagent that allows for the efficient delivery of all sizes of DNA into a broad range of cell types with extremely low cytotoxicity. FuGENE® 6 saves time and resources because it minimizes reagent preparation, media changes, and optimization. It also eliminates the side effects of toxicity while providing high transfection efficiency, which leads to successful transfection of more than 800 cell lines. FuGENE® 6 can be used for the study of gene expression, cancer biology, cell-based protein & virus production, and for many other applications in functional genomics, proteomics, and cellular analysis.
Orangu™ Cell Counting Solution Reference: OR01-1000 Non-cytotoxic: Further experiments can be carried out with the same cells Sensitive: wide linear range with low to high numbers of cells detected Easy to use: 15 minutes hands-on
Exo-spin mini-HD columns Reference: EX05-5 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.5 %) in DPBS (10x) Reference: TRY-1B10 Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
Ham’s F-12, with L-Glutamine Reference: HAM-12-A Ham's F-12 was developed for the cultivation of Chinese Hamster Ovary (CHO) cells. It is the medium of choice for supporting the growth of cells in serum-free conditions.
Newborn Calf Serum Reference: NCS-1A Newborn Calf Serum (NBCS) is collected from 3 to 10 day old calves and can be used as a high-grade replacement for Fetal Bovine Serum.
Fetal Bovine Serum, Collected in South America, Dialysed Reference: FBS-DIA-12A Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. Dialysed FBS is suitable for studies involving nutritional parameters or incorporation of labeled material.
Venor®GeM OneStep Reference: 11-8025 Similar to Venor®GeM Classic, but includes polymerase, and internal amplification control in the reaction mix.