Exo-spin standard, 8 columns, 60 ml buffer Reference: EX01-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
XerumFree Reference: XF212-0100 XerumFree is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since XerumFree is chemically defined it avoids lot-to-lot variations. All components in XerumFree are highly purified and identified chemical compounds.
cipTEC 14.4 primary cells Reference: ciPTEC14.4 CiPTEC 14.4 is a human conditionally immortalized proximal tubular epithelial cell line. Stably transfected by SV40T and hTERT, subcloned and well-characterized. Expressing proximal tubular markers alkaline phosphatase, di-peptidiyl peptidase IV and ZO-1.
Blasticidin S, Powder Reference: BLA-1X Blasticidin S is used as a selection antibiotic that inhibits protein synthesis in bacteria and eukaryotes. Transformed cells containing the resistance genes bls, bsr, or BSD can be selected.
AmnioPrime Reference: APR-B AmnioPrime is a complete ready to use medium for amniotic fluid and chorionic villi cells. It is used for karyograms, fluorescence in situ hybridization and other cytogenetic methods.
MEM Nonessential Amino Acids (100x) Reference: NEAA-B MEM Nonessential Amino Acids, also MEM NEAA, enhances cell growth and viability in several cell culture applications. These amino acids can be found in the standard MEM.
Sodium Pyruvate (100 mM) Reference: NPY-B Sodium Pyruvate is a supplement for cell culture media. It is commonly used as a carbon source in addition to glucose in a final concentration of 1mM.
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium... Reference: DMEM-HA-P10 Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
DMEM/Ham’s F-12, with L-Glutamine Reference: DMEM-12-A DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Hanks’ Balanced Salts, with Ca & Mg, w/o Phenol Red Reference: HBSS-1A Hanks' Balanced Salt Solution (HBSS) is a buffered salt solution. It is used in a variety of cell culture applications such as washing and transportation of cells or tissue preparation.
Adult Bovine Serum Reference: ABS-1A Adult Bovine Serum (ABS), collected from adult cattle, is used to supplement cell culture media. It is highly suitable for in vitro cultivation of multiple cell types.