AquaScreen® Fast Extract Reference: 32-1010 Kit for the extraction of microbial DNA from water samples. For filtration units with 47 or 50 mm frits includes filtration membranes.
Proteinase K Solution Reference: AC115 Proteinase K is a non-specific serine protease. This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.
microLYSIS Reference: 2ML-50 Release buffer, optimised to lyse cells commonly used in the lab (like bacterial (e.g.E. coli), yeast (e.g.S. cerevisiae) and human cells (e.g. HeLa) for PCRready DNA in less than 15 minutes. Supplied in 1 tube for ease of use.
iSWAB DNA Collection Kit, 1.0ml Reference: ISWAB‐DNA‐1200 DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.
25 mM dNTP Mix Reference: NUM0101 highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.
PCRbeam™ Fast PCR Detection Kit Reference: PDK0101 highQu PCRbeam™ Fast PCR Detection Kit is a convenient tool for fast detection of gene-specific amplification products obtained by PCR, LAMP or RPA. The detection is based on immunological reaction driven by Biotin and FITC (fluorescein isothiocyanate), thus the amplified DNA shall include Biotin and FITC labels. The PCR amplification has to be performed with one primer labeled with FITC at 5’-end and one primer labeled with Biotin at 5’-end. Alternatively the use of one of the labeled primers can be replaced by gene-specific FITC or Biotin labeled probe. Kit includes PCRbeam™ Membrane Strips that are coated with biotin-ligand on the test band and an anti-rabbit antibody on the control band. The bottom part of the strip which is used for sample application contains an anti-FITC antibody attached to gold particles. PCRbeam™ Detection Buffer is Tris-buffered saline enabling the detection. The PCRbeam™ Fast PCR Detection Kit can be applied for established tests or home-brew assays as a fast and sensitive yes/no detection method. The detection sensitivity is up to 100 fold higher than the one achievable with ethidium bromide stained gels what provides an environment friendly save and economical alternative to the use of mutagen stains. For establishing sensitive PCR-based tests before PCRbeam™ detection we recommend the use of hot-start PCR enzymes or master mixes, like highQu ALLin™ Hot Start Taq Mastermix or ALLin™ Hot Start Taq DNA Polymerase. Principle: The membrane strip is soaked for 10 minutes into the vial with the detection buffer mixed with PCR product. The lateral sample flow driven by gold particles moves the solution up the strip. FITC labeled DNA strand binds with the anti-FITC antibody on the gold particle and Biotin labeled DNA strand is caught by Biotin ligand attached to the test band. As both DNA strands remain hybridized at room temperature, the test band builds an aggregate that develops red-blue color. Excess gold particles that were not caught by FITC move up the strip and the anti-FITC antibody binds to the anti-rabbit antibody to develop the red-blue colored control band. If there is no PCR product in the reaction, then only the control band will be visible. If there is a specific product, the test band will be colored as well.