FuGENE® HD Transfection Reagent Reference: HD-5000 FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time.
Orangu™ Cell Counting Solution Reference: OR01-500 Non-cytotoxic: Further experiments can be carried out with the same cells Sensitive: wide linear range with low to high numbers of cells detected Easy to use: 15 minutes hands-on
Exo-spin midi columns Reference: EX04-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.05 %) in DPBS (1x) Reference: TRY-1B Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
RPMI 1640, with L-Glutamine, powder medium, with 25 mM HEPES, w/o Sodium... Reference: RPMI-HA-P50 RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate Reference: DMEM-LPXA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Mouse Serum Reference: MOU-1B Mouse Serum is obtained and pooled from normal mouse populations under strict conditions. It acts as a control or a blocking agent in binding assays.
Fetal Bovine Serum, Collected in South America, Charcoal Stripped Reference: FBS-CS-12B Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. Charcoal stripped FBS is especially useful for studying processes influenced by steroid hormones.
Internal Control DNA extra Reference: 11-9905 Additional internal amplification control for Venor®GeM qEP (11-9905)