10X Whole Blood Lysing Solution Reference: 137-10003 The 10X Whole Blood Lysing Solution is commonly used for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.
FuGENE® 6 Transfection Reagent Reference: F6-5000 FuGENE® 6 is a multi-component lipid-based transfection reagent that allows for the efficient delivery of all sizes of DNA into a broad range of cell types with extremely low cytotoxicity. FuGENE® 6 saves time and resources because it minimizes reagent preparation, media changes, and optimization. It also eliminates the side effects of toxicity while providing high transfection efficiency, which leads to successful transfection of more than 800 cell lines. FuGENE® 6 can be used for the study of gene expression, cancer biology, cell-based protein & virus production, and for many other applications in functional genomics, proteomics, and cellular analysis.
Exo-spin mini-HD columns Reference: EX05-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin (0.25 %) in DPBS (1x) Reference: TRY-2B Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
IMDM, with L-Glutamine, without Supplements Reference: IMDM-A Iscove's Modified Dulbecco's Medium (IMDM) is suitable for high density cultures. This modified DMEM formulation supports growth of different cell types such as Jurkat, COS-7 or macropahges.
Newborn Calf Serum Reference: NCS-1B Newborn Calf Serum (NBCS) is collected from 3 to 10 day old calves and can be used as a high-grade replacement for Fetal Bovine Serum.
Fetal Bovine Serum, Collected in South America, Dialysed Reference: FBS-DIA-12B Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. Dialysed FBS is suitable for studies involving nutritional parameters or incorporation of labeled material.