Alanine Transaminase Colorimetric Activity Assay Kit Reference: 700260-96 ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates. Measurement of the ALT activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing LDH. The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm.
SIRT2 Direct Fluorescent Screening Assay Kit Reference: 700280-96 The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient fluorescence-based method for screening SIRT inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate is incubated with human recombinant SIRT along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
8-Isoprostane Affinity Column Reference: 401111-1 Cayman’s 8-Isoprostane Affinity Column provides a specific and convenient means for the purification of 8-isoprostane (8-iso PGF2α) from urine and plasma samples. Each column contains 0.5 ml of affinity sorbent (mouse anti-8-isoprostane monoclonal antibody covalently bound to Sepharose 4).
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit Reference: 700300-96 Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme that catalyzes the first step in the pentose phosphate pathway. This pathway includes converting glucose to ribose-5-phosphate, a precursor to RNA, DNA, ATP, CoA, NAD, and FAD. The pathway also generates NADPH. G6PDH deficiency, the most common enzyme deficiency worldwide, causes a spectrum of diseases including neonatal hyperbilirubinemia, acute hemolysis, and chronic hemolysis. G6PDH activity has been shown to be upregulated in rat and mouse models of obesity, hyperglycemia, and hyperinsulinemia. Cayman’s Glucose-6-Phosphate Dehydrogenase Assay provides a fluorescence-based method for detecting G6PDH activity in a variety of samples including erythrocyte lysates, tissue homogenates, and cell culture samples.