SIRT2 Direct Fluorescent Screening Assay Kit

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient fluorescence-based method for screening SIRT inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate is incubated with human recombinant SIRT along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.