COX Fluorescent Activity Assay Kit Reference: 700200-2 Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP produces the highly fluorescent compound resorufin that can be analyzed using an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.
DPP (IV) Inhibitor Screening Assay Kit Reference: 700210-96 DPP (IV) inhibitors have emerged as a new class of oral antidiabetic agents. These inhibitors promote glucose homeostasis by inhibiting degradation of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) by DPP (IV). GLP-1 extends the action of insulin while suppressing the release of glucagon. Cayman’s DPP (IV) Inhibitor Screening Assay provides a convenient fluorescence-based method for screening DPP (IV) inhibitors in a 96-well format.
HDAC8 Inhibitor Screening Assay Kit Reference: 700230-96 Human HDAC8 is a class I HDAC and has been identified in a variety of human cancer tissues. Cayman’s HDAC8 Inhibitor Screening Assay provides a convenient fluorescence-based method for screening HDAC8 inhibitors. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Arg-His-Lys-Lys(e-acetyl)-AMC, is incubated with human recombinant HDAC8. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. Fluorescence is then analyzed with an excitation wavelength between 350-360 nm and an emission wavelength between 450-465 nm.