Myeloperoxidase Peroxidation Fluorometric Assay Kit Reference: 700160-2 Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and emission wavelength between 585-595 nm. The kit includes an MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluroescence.
Myeloperoxidase Inhibitor Screening Assay Kit Reference: 700170-2 Cayman’s MPO Inhibitor Screening Assay provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. Sufficient reagents are provided for a full 96-well plate assay of each type of activity.
β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit Reference: 700190-96 β-HB is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact. Pathological causes of ketosis include multiple organ failure, diabetes, childhood hypoglycemia, corticosteroid or growth hormone deficiency, intoxication with alcohol or salicylates and several inborn errors of metabolism. Cayman’s β-HB (Ketone Body) Assay provides a simple, reproducible, and sensitive tool for measuring β-HB levels in plasma, serum, or urine in a 96-well plate format with a colorimetric readout at 445-455 nm.