Prostaglandin F Receptor Reporter Assay Kit Reference: 601800-1 Cayman’s Prostaglandin F Receptor Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing the FP receptor and a CREB-regulated SEAP reporter (FP Receptor Reverse Transfection Strip Plate).
Lysosomal Staining Kit (Red Fluorescence) Reference: 601810-96 Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The lysosomal pH gradient is maintained through vacuolar ATPases which pump protons into the lysosomes. Cayman’s Lysosomal Staining Kit (Red Fluorescence) utilizes a red fluorescent dye that permeates the lysosomes based on pH gradient. Once protonated, the dye is unable to leave the lysosome resulting in enhanced fluorescence. Included in this kit is bafilomycin A1, which is an inhibitor of the vacuolar ATPase. Treatment with bafilomycin A1 results in decreased lysosomal fluorescence upon staining with the lysosomal staining reagent.
Carbamylation IP Kit Reference: 601930-1 Cayman's Carbamylation IP Kit allows for the capture and pulldown of carbamylated proteins from cell lysates using a sorbent coupled with an anti-carbamylation monoclonal antibody. The carbamylated proteins can be eluted from the resin, loaded to an SDS-PAGE gel, transferred to a nitrocellulose membrane, and probed using the included Anti-Carbamylation Polyclonal Antibody or a user-supplied target-specific antibody. A provided positive control sample assures that the assay is working.