STAT3 Transcription Factor Assay Kit Reference: 601950-96 Cayman’s STAT3 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific STAT3 DNA binding activity in nuclear extracts. As an alternative to the cumbersome electrophoretic mobility shift assay (EMSA) or the lower throughput ChIP assay, this ELISA is extremely easy to perform. This kit is an ideal way to measure STAT3 transcriptional activity downstream of cytokine or drug treatment and manipulation of cells in vitro or in vivo. Cayman’s STAT3 Transcription Factor Assay Kit detects human, mouse, rat, and non-human primate STAT3 binding on the DNA target sequence. It does not cross react with other STAT transcription factors.
DNA Laddering Kit Reference: 660990-24 Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with minimal contamination from intact chromatin. The isolated DNA is separated by electrophoresis and can be visualized using ethidium bromide.
COX Fluorescent Inhibitor Screening Assay Kit Reference: 700100-2 Cayman’s COX Fluorescent Inhibitor Screening Assay provides a convenient fluorescence-based method for screening ovine COX-1 and human recombinant COX-2 for isozyme-specific inhibitors. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP produces the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm.