NETosis Imaging Assay Kit Reference: 601750-96 ,Cayman’s NETosis Imaging Kit provides a simple staining protocol for visualizing the process of NETosis kinetically ex vivo and in vitro. A cell-permeable DNA dye allows visualization of the dynamic changes of the nucleus during the process of NETosis, while a cell-impermeable dye detects the extruded DNA. Combining the fluorescence visualization of these dyes with brightfield imaging in a high-content platform allows for exquisite differentiation between different modes of cell death. The amount of each reagent provided is sufficient for two 96-well plates.
Efferocytosis Assay Kit Reference: 601770-1 Cayman’s Efferocytosis Assay Kit employs CytoTell™ Blue to visualize effector (phagocytic) cells, CFSE to visualize bait (apoptotic) cells and staurosporine, a protein kinase C inhibitor, to induce apoptosis. The Efferocytosis Assay Kit is a convenient tool to study modulators of efferocytosis, apoptosis, pro-resolving mediators and cross-presentation. The reagents provided in this kit are sufficient to stain 5 x 107 effector cells and 2 x 108 bait cells.
Bile Acid Nuclear Receptor FXR (NR1H4) Reporter Assay Kit Reference: 601790-1 Cayman’s Bile Acid Nuclear Receptor FXR (NR1H4) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing FXR and a BSEP promoter-regulated SEAP reporter (FXR Reverse Transfection Strip Plate).