Phagocytosis Assay Kit (IgG-DyLight™ 633) Reference: 601490-1 Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-DyLight™ 633 Complex for up to 500 samples.
ROS Detection Cell-Based Assay Kit (DCFDA) Reference: 601520-96 Cayman’s ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control.
Macrophage (mouse) Elicitation Kit Reference: 601740-1 Macrophages are long-lived tissue resident cells that function to ingest and kill invading pathogens and to direct the ensuing immune responses. Cayman’s Macrophage (mouse) Elicitation Kit provides the reagents necessary for eliciting macrophages to the peritoneal cavities of up to five mice and the fluorochrome-labeled antibodies required to assess the populations recovered by flow cytometry. This kit will be useful to immunologists interested in studying monocyte/macrophage trafficking as well as those requiring a source of macrophages for downstream applications.