CBX5 (human recombinant) Reference: 11235-100 Source: Recombinant N-terminal His-tagged protein expressed in E. coli
Beclin 1 blocking peptide Reference: GTX31722-PEP Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 29 [PubMed 1927693]).[supplied by OMIM, Jul 21]
JMJD2E Strep-tagged (human, recombinant) Reference: 11237-100 Source: Active recombinant N-terminal Strep II-tagged protein expressed in E. coli • Mr: 40.8 kDa • JMJD2E catalyzes the demethylation of histone H3 at lysince residue 9. JMJD2E is an α-ketoglutarate-dependent Fe (II) oxygenase. Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity. Therefore this protein is purified by Strep-Tactin affinity chromatography.
CRMP1 blocking peptide Reference: GTX31723-PEP This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 28]
MBD2 (human recombinant, methyl binding domain aa 150-220) Reference: 11286-100 Recombinant N-terminal GST-tagged protein expressed in E. coli • MBD2 specifically binds to methylated promoters on CpG islands. MBD2 binding to 5mC facilitates the recruitment of chromatin remodeling and transcriptional repressor complexes, which results in a repressive chromatin state.
TLR5 blocking peptide Reference: GTX31724-PEP This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 29]
MeCP2 (human recombinant, methyl binding domain aa 77-166) Reference: 11287-100 Source: Recombinant N-terminal GST-tagged protein expressed in E. coli • Mr: 37 kDa • MeCP2 specifically binds to methylated promoters on CpG islands and mediates gene silencing by recruiting corepressor complexes. In vitro work suggests high affinity binding of MeCP2 is facilitated by DNA fragments containing A/T bases adjacent to the methyl-CpG.
BCAP31 blocking peptide Reference: GTX31725-PEP This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 212]
CREB-binding protein bromodomain (human recombinant) Reference: 11288-100 Source: Recombinant N-terminal GST-tagged protein expressed in E. coli • Mr: 40.8 kDa • CREBBP bromodomain has been shown to modulate the stability and function of the tumor suppressor protein p53. CREBBP bromodomain recognizes the acetylated lysine residue 382 on p53.
BCAP31 blocking peptide Reference: GTX31726-PEP This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 212]
JMJD2A-Strep tagged (human recombinant) Reference: 11299-100 Source: Active recombinant N-terminal Strep II-tagged protein expressed in E. coli • Mr: 42.7 kDa • JMJD2A catalyzes the demethylation of trimethylated forms of histone at lysine residues 9 and 36. JMJD2A is an α-ketoglutarate-dependent Fe (II) oxygenase. Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity. Therefore this protein is purified by Strep-Tactin affinity chromatography.
ASK1 blocking peptide Reference: GTX31727-PEP Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 28]