Streptavidin, Flamma® 496

 Streptavidin, Flamma® 496 is a fluorescence labeled biotin binding protein that Flamma® 496 dye is covalently attached to streptavidin. Homotetrameric protein streptavidin (53 KDa) displays high binding affinity (Kd ~ 1014 M) for biotin, and each streptavidin can bind with four biotins by a non-covalent interaction. Streptavidin has a little lower affinity than avidin but displays less non-specific binding due to near-neutral pI value and lack of carbohydrates. Flamma® 496 is a bright green fluorescent dye. Flamma® 496 might be excited using 488 nm laser line. Fluorescent streptavidin–biotin conjugation has been utilized in a wide range of applications such as cell surface labeling, ELISA, immunohistochemistry, affinity purification, FACS, EMSA, etc. The bond formation between biotin and streptavidin is rapid, and the binding is stable at high temperature, in a wide range of pH and denaturing agents. BioActs offers Streptavidin, Flamma® 496 as an effective detecting and analytic probe for diverse applications in medical diagnostics and in vitro analysis.