FAM Azide

FAM Azide is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. FAM azide couples with an alkyne to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the alkyne functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer FAM azide as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.