Flamma® 552 Isothiocyanate

Flamma® Fluors 552 Isothiocyanate is a reactive form of bright yellow dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 550/564 nm, similar to that of Alexa 555, DyLight 549, ATTO 550 and Cy3. Flamma 552 might be excited using 532, 543, 546 or 555 nm laser lines and displays excellent optical property.  Isothiocyanates are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates react to primary amine to form reasonably stable thiourea linkage. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. We offer Flamma Fluors 552 isothiocyanate for labeling of antibodies, peptides, proteins, ligands, and amplification substrates optimized for cellular labeling and detection.