BCIP/NBT Substrate

Our unique BCIP/NBT is an excellent one component chromogen substrate system that is used in Immunohistochemistry (IHC), Immunocytochemistry (ICC), western blot (WB) and in situ hybridization with alkaline phosphatase (alk phos). Alkaline phosphatase catalyzes the hydrolysis of a variety of phosphate containing substances in the alkaline pH range. This chromogen system gives purple blue color; is compatible with nuclear fast red counterstain and can be mounted with aqueous mounting medium or our ImmunoHistoMount. This chromogen can also be counterstained with our Red counter stain and mounted with mounting medium Immuno In Situ Mount.