HiScript II One Step RT-PCR Kit Reference: P611-01 HiScript® II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript® II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript® II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.Application: Reverse transcription and PCR are performed in the same tube The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments over 10 kb
HiScript II One Step RT-PCR Kit (Dye Plus) Reference: P612-01 HiScript® II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript® II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.Application: Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments up to 10 kb or more; PCR products can be directly loaded for electrophoresis
HiScript II Q RT SuperMix for qPCR Reference: R222-01 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase, which greatly improves thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II qRT SuperMix contains all the components required for reverse transcription reaction. The reaction can be performed quickly by adding template RNA and water. The volume of RNA template can be added up to the total volume It is very suitable for the reverse transcription reaction of low-concentration RNA template. 5 × HiScript® II qRT SuperMix will not freeze at -20°C and is easy to use. This product is specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. The optimized ratio of Random primers/Oligo (dT)23VN primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity and repeatability of qPCR results to the greatest extent. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis. Application: Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT)23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix is easy to use and time-saving
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) Reference: R223-01 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR (+gDNA wiper) is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the RNA template product can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. 5 × HiScript qRT SuperMix II contains all the components required for the reverse transcription reaction. Add template RNA and water to quickly react and terminate the gDNA wiper function to ensure the integrity of the cDNA. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo(dT)23VN primer mix enables cDNA synthesis to start from various regions of the RNA transcript and has the same reverse transcription efficiency, ensuring the maximum qPCR results Authenticity and repeatability. The reverse transcription product is compatible with SYBR Green and probe qPCR, and the corresponding reagents can be selected according to the purpose of the experiment to perform high-performance gene expression analysis. Application: Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT) 23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix premix is convenient and time-saving to use; gDNA wiper Mix can quickly and completely remove genome contamination
HiScript II Q Select RT SuperMix for qPCR Reference: R232-01 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q Select RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II Select qRT SuperMix contains Buffer, dNTP Mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT) can be selected as required 23VN, Random primers or gene-specific primers are used as reverse transcription primers. Oligo (dT)23VN has stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. 5 × HiScript® II Select qRT SuperMix will not freeze at -20℃, and the reaction can be performed quickly by adding template RNA and primers.The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis. Application: Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Different types of reverse transcription primers can be used flexibly for different experimental designs; Ready-to-use SuperMix premix is easy to use and time-saving
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) Reference: R233-01 This product is based on the cost-effective HiScript ® II Reverse Transcriptase and uses uniquely designed Oligo(dT) 23 VN primers to improve the specificity and sensitivity of the reaction and obtain better quality cDNA. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo (dT) 23 VN primer/Random Hexamers or gene-specific primers (GSP) can be selected according to the needs of subsequent reverse transcription experiments. At the same time, the reaction system is greatly simplified. 5 × qRT SuperMix contains all the reagents required for reverse transcription reaction except the primers and will not freeze at -20°C, making it easy to use. The 4 × gDNA wiper Mix in the kit can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. The product obtained by reverse transcription can be used in SYBR ® Green qPCR analysis method and probe analysis method for high-performance gene expression analysis.Application: Based on the efficient HiScript ® II Reverse Transcriptase; Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination
HiScript III RT SuperMix for qPCR (+gDNA wiper) Reference: R323-01 HiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the optimal buffer optimized for reverse transcription further improve the efficiency of cDNA synthesis and are suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis. Application: Easy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes; Extensive template compatibility: Compatible with RNA templates of different species and poor integrity; Super impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.; Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.
HiScript® III All-in-one RT SuperMix Perfect for qPCR Reference: R333-01 HiScript III All-in-one RT SuperMix Perfect for qPCR is an upgraded version of HiScript III RT SuperMix for qPCR(+gDNA wiper). Genomic DNA elimination and reverse transcription can be simultaneously completed, which is convenient. And it can greatly reduce contamination and RNA degradation. This kit contains HiScript III Reverse Transcriptase, heat-labile DNase and an optimized buffer system. Among them, heat-labile DNase takes effect quickly. It has high efficiency, and can be easily inactivated. The obtained cDNA can be stored stably for a long time. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis. Application: Simple and fast operation: one-step genome clearance and reverse transcription. Excellent cDNA stability: complete deactivation of thermal DNase and long-term storage of cDNA
Single Cell Sequence Specific Amplification Kit Reference: P621-01 The Single Cell Squence Specific Amplification Kit is based on a one-step RT-PCR amplification method, which is used to realize the amplification of the transcriptome in single cells or trace amounts of total RNA, so that you can uncover the expression levels of different genes between individual cells. This kit realizes that RNA extraction and purification, reverse transcription and PCR reactions are completed in the same tube, no additional operations are required, and it has the advantages of saving time, reducing experimental errors, reducing pollution, and improving sensitivity. This kit is also suitable for one-step expansion of 2-1000 cells, and the number of cycles of one-step expansion should be reduced according to the number of cells.Application: Suitable for 1-10,000 cells; One-step RT-PCR pre-amplification; Up to 500 target genes can be amplified; The amplified product is suitable for analysis by any real-time fluorescent quantitative PCR instrument; High sensitivity, low cost
miRNA Universal SYBR® qPCR Master Mix Reference: MQ101-01 This product is a special premix for miRNA quantitative reaction using SYBR® Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is based on the chemical hot-start AceTaq® DNA Polymerase and is equipped with optimized Buffer, which can greatly reduce non-specific amplification. At the same time, the special ROX Reference Dye makes the master mix suitable for all qPCR instruments without the need for different instruments To adjust the ROX concentration, just add primers and templates when preparing the reaction system to perform amplification.Application: Superior reaction system: the most suitable Buffer composition and concentration, more suitable for microRNA reverse transcription and qPCR experiments;
miRNA Universal SYBR® qPCR Master Mix Reference: MQ101-02 This product is a special premix for miRNA quantitative reaction using SYBR® Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is based on the chemical hot-start AceTaq® DNA Polymerase and is equipped with optimized Buffer, which can greatly reduce non-specific amplification. At the same time, the special ROX Reference Dye makes the master mix suitable for all qPCR instruments without the need for different instruments To adjust the ROX concentration, just add primers and templates when preparing the reaction system to perform amplification.Application: Broad platform compatibility: The addition of universal ROX to miRNA Universal SYBR® qPCR Master Mix enables mix to be compatible with qPCR instruments on different platforms without the need to adjust ROX concentration.
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) Reference: MR101-01 miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScript ® II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company's miRNA Universal SYBR ® qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.Application: Excellent linear relationship: It has a good linear relationship in a wide template interval, and can detect RNA templates as low as pg; Superior reaction system: Optimal Buffer composition and concentration, more suitable for reverse transcription of microRNA; Simple primer design: Provide matching primer design software to make primer design easier