HiScript II Q RT SuperMix for qPCR (+gDNA wiper)

HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR (+gDNA wiper) is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the RNA template product can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. 5 × HiScript qRT SuperMix II contains all the components required for the reverse transcription reaction. Add template RNA and water to quickly react and terminate the gDNA wiper function to ensure the integrity of the cDNA. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo(dT)23VN primer mix enables cDNA synthesis to start from various regions of the RNA transcript and has the same reverse transcription efficiency, ensuring the maximum qPCR results Authenticity and repeatability. The reverse transcription product is compatible with SYBR Green and probe qPCR, and the corresponding reagents can be selected according to the purpose of the experiment to perform high-performance gene expression analysis. Application: Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT) 23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix premix is ​​convenient and time-saving to use; gDNA wiper Mix can quickly and completely remove genome contamination