miRNA Universal SYBR® qPCR Master Mix

This product is a special premix for miRNA quantitative reaction using SYBR® Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is based on the chemical hot-start AceTaq® DNA Polymerase and is equipped with optimized Buffer, which can greatly reduce non-specific amplification. At the same time, the special ROX Reference Dye makes the master mix suitable for all qPCR instruments without the need for different instruments To adjust the ROX concentration, just add primers and templates when preparing the reaction system to perform amplification.Application: Superior reaction system: the most suitable Buffer composition and concentration, more suitable for microRNA reverse transcription and qPCR experiments;