Glutathione Fluorescent Detection Assay Kit Reference: 26404-96 The Glutathione Fluorescent Assay Kit is designed to measure the amount of total glutathione (GSH+GSSG) in cells and tissue homogenates and the total amount (including GSSG and protein mixed disulfides with glutathione) in plasma. The assay is simple and sensitive based on eosinGSH that emits a strong fluorescent signal detectable at 545 nm after excitation at 520 nm.[IMCO Catalog FkGSH-01]
Immuno-Oncology Screening Library Reference: 28760-50 For screening a variety of immuno-oncology associated compounds; contains more than 90 cancer and immunology-associated compounds as 10 mM stock solutions in DMSO; includes a variety of immuno-oncology target modulators, including but not limited to, adenosine, CCR, CXCR, and TLR agonists and antagonists, BTK, PI3K, and BRAF inhibitors, PD-1/PDL-1 interaction inhibitors, and HDAC inhibitors
Anti-Inflammatory Screening Library Reference: 31530-50 For screening a variety of anti-inflammatory compounds; contains ~235 compounds in a 96-well Matrix™ tube rack format as 10 mM stock solutions in DMSO; includes a variety of compounds that inhibit pro-inflammatory cytokine production, macrophage and T cell activation, cytokine storm receptor signaling, the NLRP3 inflammasome, NF-κB signaling, kinases, and inflammatory lipid mediator production and metabolism; also includes corticosteroids, glucocorticoids, and inhibitors of NETosis, coagulation, and platelet aggregation.
Lipid Droplets Fluorescence Assay Kit Reference: 500001-480 Cayman’s Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets.(17124) Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescent plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with antibodies conjugated to compatible fluorophores. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagents to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.
Phagocytosis Assay Kit (IgG FITC) Reference: 500290-1 Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.
COX (ovine) Inhibitor Screening Assay Kit Reference: 560101-96 The COX (ovine) Inhibitor Screening Assay directly measures PGF2α produced in the cyclooxygenase reaction. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, this Cayman COX Assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX (ovine) Inhibitor Screening Assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.
COX (ovine/human) Inhibitor Screening Assay Kit Reference: 560131-96 The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and reliable than an assay based on peroxidase inhibition. Cayman’s COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.
Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening... Reference: 600007-1920 PGD2 is synthesized by H-PGDS in mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. H-PGDS is therefore a key target for development of selective, potent inhibitors for therapeutic use against these diseases. Cayman’s H-PGDS FP-Based Inhibitor Screening Assay provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z' of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-PGDS with IC50 values ranging from nanomolar to millimolar concentrations.
Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening... Reference: 600007-384 PGD2 is synthesized by H-PGDS in mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. H-PGDS is therefore a key target for development of selective, potent inhibitors for therapeutic use against these diseases. Cayman’s H-PGDS FP-Based Inhibitor Screening Assay provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z' of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-PGDS with IC50 values ranging from nanomolar to millimolar concentrations.
p53 Transcription Factor Assay Kit Reference: 600020-96 The tumor suppressor protein p53 plays a crucial role in coordinating cellular responses to genotoxic stress and holds many important clinical implications in the treatment of cancer. Cayman’s p53 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double-stranded DNA (dsDNA) sequence containing the p53 response element is immobilized onto the wells of a 96-well plate. p53 contained in a nuclear extract, binds specifically to the p53 response element and is detected by addition of a specific primary antibody directed against p53. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Hydrogen Peroxide Cell-Based Assay Kit Reference: 600050-480 Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.(29448) Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.(5816) In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.(13685) Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.
Hydrogen Peroxide Cell-Based Assay Kit Reference: 600050-96 Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.(29448) Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.(5816) In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.(13685) Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.