Photosynthesis Tool Kit - quantitation Reference: AS04 051set Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies. References: Brown et al. (2007). Resource dynamics during infection of Micromonas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Rubisco quantitation kit (Western blot) Reference: AS09 409set Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.
AgriseraECL SuperBright Reference: AS16 ECL-S User Instruction Store reagents A and B in the darkness at 4-8°C.Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.Prepare your membrane prior addition of chemiluminescent substrate, by a wash with a buffer used in your protocol (PBS or TBS or TBST-T). This will allow to remove any background prior to substrate contact.Optimal visualization may be obtained up to 20 minutes after substrate contact. Usually, incubation for 2-5 is optimal.Remove excess substrate by filter paper.Cover blot with clear plastic wrap or sheet protector and expose either with x-ray film or CCD camera.In some cases Tween can quench the reaction. For best results clean containers and high quality water has to be used. HS code for this product is: 3822.00.0002.