CREB (Phospho-Ser133) Transcription Factor Assay Kit Reference: 10009846-96 CREB (cAMP-response-element-binding protein) is a transcription factor that binds to cAMP-responsive element (CRE) promoter sites to regulate the transcription of numerous genes involved in metabolic regulation, depression, long term memory, and other physiological processes. Phosphorylation on serine 133 (Ser133) activates CREB to induce transcription of target genes. Diverse stimuli such as growth factors, neurotransmitters, hypoxia, growth factors, UV light, survival signals, and stress signals are some of the known activators of CREB. Cayman’s CREB (Phospho-Ser133) Transcription Factor Assay is a non-radioactive, sensitive method for detecting CREB DNA binding activity in nuclear extracts or whole cell lysates.
Phosphatidylcholine Colorimetric Assay Kit Reference: 10009926-96 Cayman’s PC Assay provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD hydrolyzes PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.
Sphingomyelin Colorimetric Assay Kit Reference: 10009928-96 Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.
WST-8 Cell Proliferation Assay Kit Reference: 10010199-480 Cayman's WST-8 Cell Proliferation Assay provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria resulting in a water-soluble formazan which dissolves directly into the culture medium. Cayman's WST-8 assay is preferred when higher cell densities are expected, as up to 5 x 106 cells/ml can be successfully quantified.
WST-8 Cell Proliferation Assay Kit Reference: 10010199-96 Cayman's WST-8 Cell Proliferation Assay provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria resulting in a water-soluble formazan which dissolves directly into the culture medium. Cayman's WST-8 assay is preferred when higher cell densities are expected, as up to 5 x 106 cells/ml can be successfully quantified.
XTT Cell Proliferation Assay Kit Reference: 10010200-480 Cayman’s XTT Cell Proliferation Assay provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator. Reduction of XTT produces a water-soluble formazan that dissolves directly into the culture medium, eliminating the need for an additional solubilization step. This kit will allow investigators to screen drug candidates involved in cell cycle regulation.
XTT Cell Proliferation Assay Kit Reference: 10010200-96 Cayman’s XTT Cell Proliferation Assay provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator. Reduction of XTT produces a water-soluble formazan that dissolves directly into the culture medium, eliminating the need for an additional solubilization step. This kit will allow investigators to screen drug candidates involved in cell cycle regulation.
FABP4 Inhibitor/Ligand Screening Assay Kit Reference: 10010231-1 Cayman’s FABP4 Ligand Binding Assay provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a detection reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will displace the detection reagent thereby reducing the fluorescence. FABP4 is provided in high purity and in sufficient quantity for 100 tests.
Triglyceride Colorimetric Assay Kit Reference: 10010303-96 The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay Kit provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell lysates, and tissue homogenate samples. The assay is initiated with the enzymatic hydrolysis of the TGs by lipase to produce glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system with a colorimetric readout at 540 nm.
SIRT1 Direct Fluorescent Screening Assay Kit Reference: 10010401-96 The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient fluorescence-based method for screening SIRT inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate is incubated with human recombinant SIRT along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
S-Glutathionylated Protein Detection Kit Reference: 10010721-1 Glutathione (GSH) is involved in maintenance of protein sulfhydryl reduction status. PSSG may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. Cayman’s PSSG provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any PSSG adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.
Glycerol Colorimetric Assay Kit Reference: 10010755-96 Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurement of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman’s Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.