CCK-8 Cell Counting Kit Reference: A311-01 CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. . Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components. Application: Simple operation; high sensitivity; low cytotoxicity
CCK-8 Cell Counting Kit Reference: A311-02 CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. . Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components. Application: Simple operation; high sensitivity; low cytotoxicity
Dual Luciferase Reporter Assay Kit Reference: DL101-01 The Dual Luciferase Reporter Assay Kit is used to detect the fluorescence intensity of the Luciferin substrate after the reporter plasmid is transfected into the cells, so as to reflect the expression of luciferase and achieve the purpose of detecting gene regulation. First, use luciferin as a substrate to detect Firefly luciferase, and then use coelenterazine as a substrate to detect Renilla luciferase (Renilla luciferase) to achieve dual luciferase reporter genes Detection. The dual luciferase detection kit detects the activity of firefly luciferase to evaluate the effect of regulatory elements induced by stimuli; Renilla luciferase is used as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells To ensure reliable results.Application: The fluorescent signal is strong; Wide detection range; high sensitivity
Add&Read Human IgG Kit Reference: DD2101-01 Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG. Application: Simple steps Has good compatibility Quantitatively accurate
Add&Read Human IgG Kit Reference: DD2101-02 Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG. Application: Simple steps Has good compatibility Quantitatively accurate
Add&Read Human Fc Kit Reference: DD2102-01 Add&ReadTM Human Fc Kit can be used to detect the concentration of Human IgG or hFc-fusion protein (hFc-fusion protien) in the cell supernatant or after purification. There is an antibody in the kit that recognizes the Fc region of Human IgG and marks the fluorescent donor Eu (anti-human IgG-Eu); and Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, anti-human IgG-Eu binds to Human IgG-A2, 320 nm light excites fluorescence donor Eu, Eu donor emits 620 nm light, this 620 nm light excites fluorescent acceptor A2, A2 acceptor It emits 665 nm light, which is the phenomenon of fluorescence resonance energy transfer (FRET). When the human IgG or hFc-fusion protien to be tested is added to the solution, it will compete to bind anti-human IgG-Eu and destroy the FRET phenomenon. The concentration of Human IgG or hFc-fusion protien to be tested is inversely proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG, hFc-fusion protein or bispecific antibodies. Application: Simple steps Has good compatibility Quantitatively accurate
Add&Read Human Fc Kit Reference: DD2102-02 Add&ReadTM Human Fc Kit can be used to detect the concentration of Human IgG or hFc-fusion protein (hFc-fusion protien) in the cell supernatant or after purification. There is an antibody in the kit that recognizes the Fc region of Human IgG and marks the fluorescent donor Eu (anti-human IgG-Eu); and Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, anti-human IgG-Eu binds to Human IgG-A2, 320 nm light excites fluorescence donor Eu, Eu donor emits 620 nm light, this 620 nm light excites fluorescent acceptor A2, A2 acceptor It emits 665 nm light, which is the phenomenon of fluorescence resonance energy transfer (FRET). When the human IgG or hFc-fusion protien to be tested is added to the solution, it will compete to bind anti-human IgG-Eu and destroy the FRET phenomenon. The concentration of Human IgG or hFc-fusion protien to be tested is inversely proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG, hFc-fusion protein or bispecific antibodies. Application: Simple steps Has good compatibility Quantitatively accurate
BCA Protein Quantification Kit Reference: E112-01 The BCA Protein Quantification Kit is currently one of the most sensitive protein determination methods. Under alkaline conditions, the protein reduces Cu2+ to Cu+, and Cu+ interacts with the unique BCA Reagent A (containing BCA). The reaction produces a sensitive color reaction and forms a purple complex. The water-soluble complex shows strong absorbance at A562 nm, and the absorbance and protein concentration have a good linear relationship in a wide range. The absorbance can be calculated Protein concentration. Therefore, use a microplate reader to measure its absorbance at A562 nm and compare it with the standard curve to calculate the concentration of the protein to be tested. Commonly used detergents such as SDS, Triton X-100, Tween-20 do not affect the test results, but are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. In the experiment, if the background value of the sample dilution or lysate itself is found to be high, the Bradford protein concentration determination kit can be used. Application: Simple operation; high sensitivity; wide compatibility; standard curve with high linear relationship
BCA Protein Quantification Kit Reference: E112-02 The BCA Protein Quantification Kit is currently one of the most sensitive protein determination methods. Under alkaline conditions, the protein reduces Cu2+ to Cu+, and Cu+ interacts with the unique BCA Reagent A (containing BCA). The reaction produces a sensitive color reaction and forms a purple complex. The water-soluble complex shows strong absorbance at A562 nm, and the absorbance and protein concentration have a good linear relationship in a wide range. The absorbance can be calculated Protein concentration. Therefore, use a microplate reader to measure its absorbance at A562 nm and compare it with the standard curve to calculate the concentration of the protein to be tested. Commonly used detergents such as SDS, Triton X-100, Tween-20 do not affect the test results, but are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. In the experiment, if the background value of the sample dilution or lysate itself is found to be high, the Bradford protein concentration determination kit can be used. Application: Simple operation; high sensitivity; wide compatibility; standard curve with high linear relationship
KRISHZYME™ Heparin Factor IIa Assay Kit Reference: KBBA03S Chromogenic assay for testing Heparins (UFH) in purified systems by measurement of Factor IIa inhibition, in compliance with EP Pharmacopoeia. About the kit: Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity, Recovery rates are between 85 - 115%, Ready to use with a standard protocol with break-apart pre-coated wells, Validated as per US FDA guidelines for Bioassays, Optimized for matrix effects to ensure higher sensitivity, Shelf life: 1 year. The inhibitory effect of Anti-Thrombin III (AT-III) on thrombin (Factor IIa) and other coagulation serine proteases in plasma is increased several thousand-fold by heparin. This inhibition accounts for the anticoagulant effect of heparin. The quantitative determination of heparin levels by the measurement of their Anti-IIa activity is a necessary tool for monitoring treatment efficacy. Unfractionated heparin (UFH) catalyzes both reactions equally. The Factor IIa inhibition test is the most useful assay covering the widest variety of heparin preparations. In the assay, the rate of Factor IIa inhibition is directly proportional to the heparin concentration since both Factor IIa and AT-III are in excess. The residual factor IIa activity is inversely proportional to the heparin concentration.