Add&Read Human IgG Kit

Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG. Application: Simple steps Has good compatibility Quantitatively accurate