Category: Research kits

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  • Brand: ApexBio
  • Brand: Invent
  • Brand: SignalChem Diagnostics
  • Brand: Vazyme
Reference: A311-01

CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. . Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components. Application: Simple operation; high sensitivity; low cytotoxicity

Reference: A311-02

CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. . Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components. Application: Simple operation; high sensitivity; low cytotoxicity

Reference: DL101-01

The Dual Luciferase Reporter Assay Kit is used to detect the fluorescence intensity of the Luciferin substrate after the reporter plasmid is transfected into the cells, so as to reflect the expression of luciferase and achieve the purpose of detecting gene regulation. First, use luciferin as a substrate to detect Firefly luciferase, and then use coelenterazine as a substrate to detect Renilla luciferase (Renilla luciferase) to achieve dual luciferase reporter genes Detection. The dual luciferase detection kit detects the activity of firefly luciferase to evaluate the effect of regulatory elements induced by stimuli; Renilla luciferase is used as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells To ensure reliable results.Application: The fluorescent signal is strong; Wide detection range; high sensitivity

Reference: DD2101-01

Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG. Application: Simple steps Has good compatibility Quantitatively accurate

Reference: DD2101-02

Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG. Application: Simple steps Has good compatibility Quantitatively accurate