JC-1 Mitochondrial Membrane Potential Assay Kit Reference: 10009172-100 Mitochondrial membrane potential, (ΔψM), is an important parameter of mitochondrial function used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial (ΔψM), JC-1 spontaneously forms complexes known as J-aggregates with intense red fluorescence. On the other hand, in apoptotic or unhealthy cells with low (ΔψM), JC-1 remains in the monomeric form, which shows only green fluorescence. Cayman’s JC-1 Mitochondrial Membrane Potential Assay Kit provides all the necessary reagents, as well as complete instructions, for analysis of mitochondrial integrity in whole cells. The assay is applicable to fluorescence microscopy and fluorescent plate reader. For flow cytometry, Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit (Item No. 701560) is recommended.
Malachite Green Phosphate Assay Kit Reference: 10009325-2 Cayman’s Malachite Green Phosphate Assay provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm. Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates. This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications. The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays.
Cell Cycle Phase Determination Kit Reference: 10009349-100 Cayman’s Cell Cycle Phase Determination provides an easy to use tool for studying the induction and inhibition of cell cycle progression in any cell suspension sample. The assay involves the fixation and permeabilization of the cells of interest, making possible the staining of DNA within intact cells by propidium iodide. This kit will allow the investigator to determine the percentage of cells in a given sample that are within G1/G0, G2, or S phase at the time of fixation, as well as to quantify cells in the sub-G1 phase prior to apoptosis by FC.