WST-1 Cell Proliferation Assay Kit Reference: 10008883-480 Cayman’s WST-1 Proliferation Assay is based on the reduction of tetrazolium salt WST-1 to a soluble formazan dye by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation.
TBARS Assay Kit Reference: 10009055-96 Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from PUFAs results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these TBARS is a well-established method for screening and monitoring lipid peroxidation. Cayman’s TBARS Assay provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates and cell lysates.
Caspase-3/7 Fluorescence Assay Kit Reference: 10009135-96 Cayman’s Caspase-3/7 Fluorescence Assay Kit employs a specific substrate, N-Ac-DEVD-N’-MC-R110, which upon cleavage by active caspase-3 or caspase-7, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of caspase-3 or -7. Active caspase-3 is included in the kit for use as a positive control. The caspase-3/7 inhibitor, N-Ac-DEVD-CHO, is also included in the kit for verifying the specificity of the substrate.