Protein Carbonyl Fluorometric Assay Kit Reference: 701530-96 Cayman’s Protein Carbonyl Fluorometric Assay Kit utilizes the rhodamine B hydrazide (RBH) reaction to measure the protein carbonyl content in plasma, serum, cell lysates, or tissue homogenates in a convenient 96-well format. Formation of the fluorescent protein carbonyl-RBH hydrazone is analyzed using an excitation wavelength of 560 nm and an emission wavelength of 585-595 nm.
JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit Reference: 701560-500 Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in ΔψM, reflected by aggregation level of JC-1, can be determined as a ratio of red to green mean fluorescence intensities using flow cytometry. Flow cytometry is an ideal way to assess the JC-1 aggregation at the single-cell level, and provides the added benefit of potential to multiplex other readouts from the same cells.3 This kit additionally contains FCCP for treatment of cells as a compensation control. The reagents in this kit are sufficient for staining up to 500 samples for JC-1.
Mitochondrial ROS Detection Assay Kit Reference: 701600-96 As one of the major sources of reactive oxygen species (ROS) in the cell, mitochondrial ROS has often been difficult to single out from the other sources of ROS within the cell. While some ROS dyes such as DHE and DCFDA tend to be less location-specific and dyes such as ADHP are only extracellular, Mitochondrial ROS Detection Reagent is targeted primarily to the mitochondrion making Mitochondrial ROS Detection Reagent an ideal reagent to study mitochondrial ROS.(32281,4059,24620)