PAD1 Inhibitor Screening Assay Kit (Ammonia) Reference: 701450-96 Cayman’s PAD1 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD1 inhibitors. PAD1 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar kinetic properties to the natural substrates, producing ammonia. Ammonia reacts with a detector resulting in a fluorescent product. Fluorescence is then analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.
PAD3 Inhibitor Screening Assay Kit (Ammonia) Reference: 701470-96 Cayman’s PAD3 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD3 inhibitors. PAD3 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar kinetic properties to the natural substrates, producing ammonia. Ammonia reacts with a detector resulting in a fluorescent product. Fluorescence is then analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.
DUB Activity Assay Kit Reference: 701490-96 Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. Each kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.