TBARS (TCA Method) Assay Kit Reference: 700870-96 Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such as malondialdehyde (MDA). MDA can be quantified through a controlled reaction with thiobarbituric acid, generating 'Thiobarbituric Acid Reactive Substances' (TBARS). Cayman’s TBARS (TCA Method) Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates. The MDA-TBA adduct formed by the reaction of MDA and TBA under high temperature (90-100°C) and acidic conditions can be measured either colorimetrically at 530-540 nm or with much higher sensitivity fluorometrically at an excitation wavelength of 530 nm and an emission wavelength of 550 nm.
MitoCheck® Complex I Activity Assay Kit Reference: 700930-96 Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck® Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I.
MitoCheck® Complex II Activity Assay Kit Reference: 700940-96 Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately, oxidation of succinate will lead to reduction of O2, the terminal step in mitochondrial respiration. Cayman’s MitoCheck® Complex II Activity Assay allows for the activity of complex II to be determined without the need to isolate mitochondria or pre-incubate with antibodies. As complex II oxidizes succinate, electrons are passed to an analog of ubiquinone and then on to DCPIP, which, when oxidized, absorbs in the 600 nm range. The absorbance of DCPIP will decrease upon reduction. Thus, Complex II activity is measured as a decrease in absorbance at 600 nm over time.