β-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit Reference: 700740-96 β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a reliable index of the level of normal ketosis as well as detect pathological ketoacidosis, including the assessment of the severity of diabetic coma and monitor insulin requirements in diabetic patients. Cayman’s β-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying β-HB from plasma, serum, urine, tissue homogenates, and cell culture samples. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase. Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the β-HB concentration.
Glucose-6-Phosphate Fluorometric Assay Kit Reference: 700750-96 Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling protection against oxidative damage. Disruption of G6P activity leads to glycogen storage disease type I or von Gierke’s disease, a group of inherited metabolic diseases characterized by severe hypoglycemia, growth retardation, and hepatomegaly, due to accumulation of glycogen and fat in the liver. Cayman’s Glucose-6-Phosphate Fluorometric Assay provides a fluorescence-based method for detecting G6P in tissue homogenates and cell culture samples. In the assay, G6PDH catalyzes the oxidation of G6P to 6-phospho-D-gluconate, along with the concomitant reduction of NADP+ to NADPH. NADPH reacts with the fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Aldehyde Dehydrogenase Activity Assay Kit Reference: 700800-96 Aldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological functions. Pharmacological inhibitors or activators of ALDH activity have been developed to examine the metabolism of alcohol as well as a number of pathological conditions, including cancer, Type II hyperprolinemia, Sjögren-Larsson Syndrome, Parkinson’s Disease, Cardiac Disease, and hyperammonemia. Cayman’s Aldehyde Dehydrogenase Assay provides a fluorescence-based method for detecting ALDH activity in tissue homogenates, cell culture samples, and purified ALDH preparations. In the assay, ALDH catalyzes the oxidation of acetaldehyde to acetic acid, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.