Neutrophil Elastase Activity Assay Kit Reference: 600610-2 Neutrophil elastase is stored within cytoplasmic azurophilic granules in the neutrophil and released upon stimulation by pathogens where it acts either as free protein or is associated with networks of extracellular traps (NET). Together with other proteases released from activated neutrophils, neutrophil elastase plays a critical role in degrading invading pathogens and thus provides the earliest line of defense in the immune system. It is thought to play a critical role in tumor invasion and metastasis as well as other inflammatory conditions. Cayman’s Neutrophil Elastase Activity Assay kit is designed to be used to study compounds regulating elastase release in neutrophils. The kit employs a specific non-fluorescent elastase substrate, (Z-Ala-Ala-Ala-Ala)2Rh110, which is selectively cleaved by elastase to yield the highly fluorescent compound R110, which can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate elastase release from neutrophils.
PPARγ-LBD Ligand Screening Assay Kit Reference: 600616-384 Cayman’s PPARγ-LBD Ligand Screening Assay Kit provides a convenient fluorescence polarization (FP)-based single-step assay for screening ligands of the PPARγ-Ligand Binding Domain (PPARγ-LBD). In this assay, a ligand of PPARγ is conjugated to fluorescein and is used as the displacement probe. Ligands, agonist, and antagonists of PPARγ-LBD will displace the fluorescent probe leading to a decrease in FP.
Neutrophil Myeloperoxidase Activity Assay Kit Reference: 600620-2 Myeloperoxidase (MPO), released from neutrophils to degrade invading pathogens, provides one of the earliest lines of defense in innate immunity. It catalyzes the hydrogen peroxide-mediated oxidation of halide ions to products such as hypochlorous acid (HOCl). These reactive products participate in a variety of secondary reactions which in turn lead to modifications of proteins and destruction of the extracellular matrix. Cayman’s Neutrophil Myeloperoxidase Activity Assay kit utilizes 3,3',5,5'-tetramethyl-benzidine (TMB) as a chromogenic substrate, which upon reacting with MPO, yields a blue color detectable by its absorbance at 650 nm. The color intensity is proportional to the amount of MPO in the sample. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate MPO release from neutrophils. A specific inhibitor for MPO, 4-aminobenzhydrazide (ABH), is also included in the kit for verifying the specificity of the assay.