EP4 Receptor (rat) Activation Assay Kit (cAMP) Reference: 600410-1 The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Activation Assay Kit (cAMP) consists of a 96-well plate coated with a transfection complex containing a DNA construct for rat EP4 Receptor (EP4 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 stimulates cAMP generation and increases intracellular cAMP levels, which can be measured by a competitive EIA using the Mouse Anti-Rabbit IgG Coated Plate and the reagents included in the kit. An EP4 agonist, PGE2, is included in the kit as a positive control.
Cholesterol Uptake Cell-Based Assay Kit Reference: 600440-1 Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. The mechanisms controlling cholesterol absorption are important to understand for the prevention and treatment of hypercholesterolemia. Cayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, a cholesterol intracellular trafficking inhibitor, is included as a positive control.
Glycolysis Cell-Based Assay Kit Reference: 600450-1 Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium, and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers. ,