SPE Cartridges (C18) (1 ml) Reference: 400077-5 This product may be used for purification of various lipid mediators such as prostaglandins and hydroxylated fatty acids from various sample types prior to analysis by LC-MS/MS or ELISA. References are provided below for applicable C18 sample purification methods prior to LC-MS analysis.(19261,26615,8825,15162) For sample purification prior to analysis by ELISA, we recommend consulting the appropriate assay kit booklet for instructions.
EP4 Receptor (rat) Reporter Assay Kit Reference: 600350-100 The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP4 receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP4 Receptor Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP4. PGE2 is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.
Glutathione Cell-Based Detection Kit (Blue Fluorescence) Reference: 600360-96 Glutathione (GSH) is a low molecular-weight tripeptide thiol that serves as a primary cellular anti-oxidant and plays a fundamental role in the elimination of environmental toxins. During early apoptosis, cells may exclude GSH, causing a decrease in intracellular GSH levels. The intracellular level of GSH is thus used as an indicator of cell health. Cayman’s Cell-Based Glutathione Assay Kit (Blue Fluorescence) employs a cell-permeable dye, monochlorobimane (MCB), which reacts with GSH to generate a highly fluorescent product that can be measured using excitation and emission wavelengths of 380 and 480 nm, respectively. The kit can be easily adapted to screening programs for therapeutic compounds regulating intracellular GSH levels.
ALP Assay Buffer Reference: 400083-30 ALP Assay Buffer has been tested and formulated to work exclusively with Cayman’s Alkaline Phosphatase Colorimetric Activity Assay Kit (Item No. 701710).
Multidrug Resistance Assay Kit (Calcein AM) Reference: 600370-1 Cayman's Multidrug Resistance Assay Kit provides a convenient tool for studying the modulation of cellular multidrug resistance (MDR) machinery. The kit employs calcein AM, a cell-permeable nonfluorescent dye, which is cleaved intracellularly to yield the impermeant fluorescent molecule, calcein. This calcein is rapidly excluded from cells expressing the MDR proteins P-gp or MRP, and so functions as a probe for the detection of chemical compounds inhibiting MDR proteins. Propidium iodide is provided as a stain for marking dead or damaged cells. Cyclosporin A, a competitive inhibitor of MDR proteins, and verapamil, a noncompetitive inhibitor, are included as controls.