Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) Reference: K1024-1 Inhibit proteolytic degradation during bacterium lysis and protein extraction
Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) Reference: K1024-20 Inhibit proteolytic degradation during bacterium lysis and protein extraction
Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) Reference: K1024-50 Inhibit proteolytic degradation during bacterium lysis and protein extraction
Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) Reference: K1024-100 Inhibit proteolytic degradation during bacterium lysis and protein extraction
Broad Spectrum Protease Inhibitor Cocktail (EDTA free) (100x) Reference: AR1182-1 Boster’s Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.
ECL Plus Western Blotting Substrate Reference: AR1196-200 Boster's ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). Boster Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film.
Plus RIPA Lysis Buffer Reference: AR0102-100 Plus RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. RIPA lysis buffer is highly compatible with various downstream protein analysis applications.
4% Paraformaldehyde (PFA) Solution in PBS Reference: AR1068 4% Paraformaldehyde solution in PBS (with DEPC) is a ready-to-use solution for sample preparation and fixing cells for immunohistochemistry (IHC).
Citrate Buffer Powder Reference: AR0024 Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining Reference: AR1176 DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5·2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be seen under the microscope after staining. The largest excitation wavelength for DAPI is 340nm (ultraviolet), and the largest emission wavelength is 488nm (blue). When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm, while the largest emission wavelength becomes 460nm. DAPI's blue emission makes it suitable for combined assays where the fluorescence ranges of DAPI and other IHC-employed fluorescent molecules like green-fluorescent fluorescein and GFP, or red-fluorescent stains like Texas Red, are completely distinctive.
Nuclear Fast Red Reference: AR0008 Nuclear Fast Red Solution, Counterstain in IHC (BCIP/NBT chromogenic reaction)
Broad Spectrum Protease Inhibitor Cocktail (100x) Reference: AR1182 Broad Spectrum Protease Inhibitor Cocktail; for inhibiting proteases and esterase.