Trident 20% SDS (w/v) Reference: GTX16370 SDS Solution is sodium dodecyl sulphate in milli-Q water. SDS is a detergent known to denature proteins. It is commonly used in polyacrylamide gel electrophoresis for protein separation. It is also used in nucleic acid extraction procedures for the disruption of cell walls and dissociation of nucleic acid:protein complexes.
Trident Blue Prestained Protein Ladder Reference: GTX16376 The Trident Blue Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 1 to 18 kDa. Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer). The Trident Blue Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, veri?cation of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Trident pico Western HRP Substrate Reference: GTX17435-S Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.
Trident pico Western HRP Substrate Reference: GTX17435 Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.
AP Enhancer Reference: GTX20671 Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization, and DNA sequencing. Detection of this enzyme requires the generation of an insoluble colored reaction end product. The stronger the signal, the better the staining. This is a new stable solution to increase the signal generated by alkaline phosphatase several fold. A single treatment of tissue sections with enhancer before the treatment with substrate/chromogen enables the visualization of difficult to localize antigens. This enhancer works well with Fast Red but can be used with BCIP/NBT and BCIP/INT also. Specimens stained with the above substrates/chromogens can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.
Peroxidase conjugate stabilizer Reference: GTX20673 Peroxidase is one of the most widely used enzyme markers in the immunoassay systems. Because of the higher titre of labeled enzymes, diluted reagent has a tendency to start losing its activity after a few weeks, even at 4ºC. Peroxidase-conjugate diluent is specially designed to solve this problem. Labeled peroxidase when diluted in this diluent will not lose its activity. This diluent will stabilize the peroxidase conjugate and will increase its shelf life for several years when stored at 4ºC. This Peroxidase diluent/stabilizer contains immunologically inert protein to further stabilize the protein peroxidase conjugate.
DAB Enhancer Reference: GTX20675 DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
Antigen Retrieval Solution Reference: GTX20970 Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product used to be called ""Carezyme Trypsin Enzymatic Antigen Retrieval Solution"".
AP chromogen BCIP/NBT (ready-to-use) Reference: GTX27468 Alkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.
Antigen retrieval condition-Pepsin (ready to use) Reference: GTX28194 Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S1 protein.
Clear Mount mounting medium-general purpose (Ready-to-use) Reference: GTX28213 Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents. Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.