CheKine™ Superoxide anion Scavenging Capacity Assay Kit

CheKine™ Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2－) is provided by xanthine oxidase (XO) catalyzed reaction. O2－ reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2－ thus less O2－ is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.