Recombinant Human Kelch-like protein 12(KLHL12)

Substrate-specific adapter of a BCR E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport . The BCR complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR acts by mediating monoubiquitination of SEC31. The BCR complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR complex and SEC31, promoting monoubiquitination of SEC31 and subsequent collagen export. As part of the BCR complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins .