Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.)

IL-1 is a group of inflammatory cytokines, represented by interleukin-1alpha and interleukin-1beta. IL-1Ra is a cytokine initially isolated from macrophages. It shares 19% of homologous amino acids with IL1A and 30% of homologous amino acids with IL1B. IL-1Ra is abundant in the proximal digestive tract reproductive system, skin, bone marrow, and liver. IL-1Ra is the principal endogenous IL-1 antagonist. IL-1Ra is upregulated during host acute or chronic inflammatory responses. IL-1Ra plays diverse roles in anti-inflammatory and anti-tumor processes, either inhibiting tumor angiogenesis and proliferation or suppressing tumor migration and metastasis. The protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) is produced by using two different vectors, one encoding for the Fc Knobs (LALA-PG) (human):IL-1Ra (mouse) sequence (synthesizing a protein of 50kDa) and one encoding for the Fc Holes (LALA-PG) sequence (synthesizing a protein of 30kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.). This Fc-KIH format allows our mouse IL-1Ra protein to form a monomer that is the most active structure to bind and inhibit the IL-1 receptor. The LALA-PG mutations inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.