Quote T4 DNA polymerase
In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.Application: Connect the sticky end or blunt end quickly in 5 minutes at room temperature;
T/A cloning;
dsDNA cut repair
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Reference:
N101-01
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