Quote BsaI
BsaI is designed to be used upstream of the in vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. As one of the type IIS restriction enzymes, it recognizes asymmetric DNA sequences and cleaves outside of their recognition sequence.
The cleavage site of BsaI is GGTCTC (N1/N5), where the GGTCTC acts as the recognition site, and the N1/N5 represents the cleavage site with a 5' overhang. Kactus provides a BSA-free reaction buffer to ensure the safety of mRNA vaccine production.
Read moreThe cleavage site of BsaI is GGTCTC (N1/N5), where the GGTCTC acts as the recognition site, and the N1/N5 represents the cleavage site with a 5' overhang. Kactus provides a BSA-free reaction buffer to ensure the safety of mRNA vaccine production.
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Reference:
GMP-BSA-EE101
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