T7 RNA Polymerase

T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7 RNA Polymerase initiates transcription at the G* in the classic T7 promoter-taatacgactcactataG*GG. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended. Note: The cofactor Mg2+ is required for the synthesis. Restriction enzymes that produce blunt ends or 5'-overhangs are recommended.