Quote RNase R
RNase R is an E. coli exoribonuclease that exhibits 3’-to- 5’ exonuclease activity, efficiently digesting nearly all linear RNA species but does not digest lariat or circular RNA structures or double-stranded RNA with 3 ́ overhangs shorter than seven nucleotides. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA, and intron lariats. RNase R is often used in alternative splicing for the isolation of circular or lariat RNA from a mixture of total RNA.
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Reference:
RNR-EE001
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