UniPeak U+ One Step RT-qPCR SYBR Green Kit

UniPeak U+ One Step RT-qPCR SYBR Green Kit is a dye-based RT-qPCR reagent specifically designed for quantitative PCR detection using RNA as the template. The dye-based fluorescence quantitative detection presents two major challenges: the difficulty of detecting low-concentration samples and the issue of non-specific amplification. This product addresses these challenges with a next-generation antibody-modified Taq DNA Polymerase and a one-step, temperature-activated Reverse Transcriptase, combined with an optimized buffer system for RT-qPCR, offering enhanced sensitivity and specificity. To further ensure accurate quantification, a dUTP/UDG anti-contamination system is included, eliminating aerosol contamination at room temperature. Additionally, the 5 × gDNA Wiper effectively removes any residual genomic DNA contamination from RNA templates. The 2 × One Step SYBR Green Master Mix includes tracking dye and universal ROX Reference Dye, making it compatible with all types of qPCR instruments, requiring only the addition of primers and templates for amplification.