FreeZol Reagent

FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal  tissues, and simple plant tissues. Compared with the conventional Trizol method, this product  features a simple procedure that can be performed at room temperature with no need to use  chloroform for phase separation. In addition, this product ensures the integrity and purity of  the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the  organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be  completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.

Features  

Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanol

Ease to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperature

High RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method