Effect Diluent Kit, Animal-free

They can be used for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. Effect Diluents, Animal-free efficiently minimize matrix effects, cross-reactions and unspecific binding in immunoassays like ELISA, Western blotting, Immunohistochemistry, protein arrays and immuno-PCR.

The Effect Diluents, Animal-free are used alternatively to the standard sample or antibody dilution buffers:

• In ELISA for the dilution of specimen and detection antibodies.
• In Western Blotting for the dilution of primary and secondary antibodies.
• In Protein arrays for the dilution of specimen and detection antibodies.
• In immuno-PCR as a washing buffer.

Three versions of the diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g., by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.