Anti-Topoisomerase II alpha Mouse mAb

DNA topoisomerases I and II are a class of nucleases, with type II including two highly homologous isozymes: topoisomerase IIα and IIβ. These enzymes are capable of regulating the topological structure of DNA, maintaining genome integrity, and playing important roles in DNA replication, recombination, transcription, and chromosome segregation. Topoisomerase I can cleave and reconnect one strand of doublestranded DNA, while topoisomerase II can temporarily break the double-stranded DNA and reconnect the broken ends. Topoisomerases are highly sensitive to various stresses. Under acidic pH or oxidative stress, topoisomerases can convert into DNA cleavage nucleases, leading to genomic instability and cell death. Certain topoisomerase-targeting drugs for DNA damage, such as etoposide, can also convert topoisomerases into nucleases. These enzymes are typically trapped as an intermediate product, capable of covalently binding to the 5' end of the cleaved DNA chain. Research has shown that this intermediate product can cause genomic instability and cell death. Therefore, targeting topoisomerases has become an important direction in the development of cancer chemotherapy drugs._x000D_