KRIBIOLISA™ HEK HCP ELISA

Immunoassay for the measurement of HEK Host Cell Proteins. The KRIBIOLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA procedure. Samples containing HEK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-HEK antibody in microtiter strips coated with an affinity purified capture anti-HEK antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HEK HCPs present.